NAPG Antikörper (AA 2-185)

Produktnummer ABIN1742244

Produktdetails anti-NAPG Antikörper

Target: NAPG (N-Ethylmaleimide-Sensitive Factor Attachment Protein, gamma (NAPG))

Bindungsspezifität: AA 2-185

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NAPG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Spezifität: Specific for gamma SNAP.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide AAQKINEGLEHLAKAEK (2-18 in rat gamma SNAP) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 500 up to 1 : 2000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Referenzen für anti-NAPG Antikörper (ABIN1742244)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Burgalossi, Jung, Meyer, Jockusch, Jahn, Taschenberger, OConnor, Nishiki, Takahashi, Brose, Rhee: "SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming." in: Neuron, Vol. 68, Issue 3, pp. 473-87, (2010) (PubMed).

Details zu NAPG

Target: NAPG (N-Ethylmaleimide-Sensitive Factor Attachment Protein, gamma (NAPG))

Andere Bezeichnung: gamma SNAP (NAPG Produkte)