SNAP25 Antikörper (AA 192-206)

Produktnummer ABIN1742235

Dieses Anti-SNAP25-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SNAP25 in WB, ELISA, IHC, IP und ICC. Geeignet für Human, Ratte, Maus, Huhn, Zebrafisch (Danio rerio), Hamster und Goldfisch. Dieses Primary Antibody wurde in 9+ Publikationen zitiert.

Kurzübersicht für SNAP25 Antikörper (AA 192-206) (ABIN1742235)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reaktivität: Human, Ratte, Maus, Huhn, Zebrafisch (Danio rerio), Hamster, Goldfisch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SNAP25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-SNAP25 Antikörper

Bindungsspezifität: AA 192-206

Spezifität: Specific for SNAP 25.

Aufreinigung: antiserum

Immunogen: Synthetic peptide IDEANQRATKMLGSG (aa 192-206 in human SNAP 25) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 10000 (AP staining)
ICC: 1 : 100

Kommentare:

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS

Handhabung: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-SNAP25 Antikörper (ABIN1742235)

Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).

Fossati, Morini, Corradini, Antonucci, Trepte, Edry, Sharma, Papale, Pozzi, Defilippi, Meier, Brambilla, Turco, Rosenblum, Wanker, Ziv, Menna, Matteoli: "Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis." in: Cell death and differentiation, Vol. 22, Issue 9, pp. 1425-36, (2015) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Young, Franciosi, Spreeuw, Deng, Sanders, Tam, Huang, Singaraja, Zhang, Bissada, Kay, Hayden: "Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice." in: PLoS ONE, Vol. 7, Issue 5, pp. e36315, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Camoletto, Vara, Morando, Connell, Marletto, Giustetto, Sassoè-Pognetto, Van Veldhoven, Ledesma: "Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18." in: PLoS ONE, Vol. 4, Issue 4, pp. e5310, (2009) (PubMed).

Johansson, Ericsson, Janson, Beraki, Stanić, Mandic, Wikström, Hökfelt, Ogren, Rozell, Berggren, Bark: "An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function." in: PLoS genetics, Vol. 4, Issue 11, pp. e1000278, (2008) (PubMed).

Details zu SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Andere Bezeichnung: SNAP 25

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport