RAB3A Antikörper (AA 2-14)

Produktnummer ABIN1742211

Dieses Anti-RAB3A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RAB3A in WB, ELISA, IHC, ICC und IP. Geeignet für Human, Ratte, Maus, Zebrafisch (Danio rerio), Rind (Kuh) und Hamster. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für RAB3A Antikörper (AA 2-14) (ABIN1742211)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reaktivität: Human, Ratte, Maus, Zebrafisch (Danio rerio), Rind (Kuh), Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-RAB3A Antikörper

Bindungsspezifität: AA 2-14

Spezifität: Specific for Rab 3a.

Aufreinigung: antiserum

Immunogen: Synthetic peptide ASATDSRYGQKES (aa 2-14 in rat Rab 3a) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: 1 : 1000

Kommentare:

ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS

Handhabung: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-RAB3A Antikörper (ABIN1742211)

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Rastaldi, Armelloni, Berra, Li, Pesaresi, Poczewski, Langer, Kerjaschki, Henger, Blattner, Kretzler, Wanke, DAmico: "Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a." in: The American journal of pathology, Vol. 163, Issue 3, pp. 889-99, (2003) (PubMed).

Schlüter, Khvotchev, Jahn, Südhof: "Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40919-29, (2002) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Details zu RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Andere Bezeichnung: Rab 3a

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport