RAB3A Antikörper (AA 154-227)

Produktnummer ABIN1742210

Der Kaninchen Polyklonal Anti-RAB3A-Antikörper wurde für WB, IHC und ICC validiert. Er ist geeignet, RAB3A in Proben von Human, Ratte und Maus zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für RAB3A Antikörper (AA 154-227) (ABIN1742210)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-RAB3A Antikörper

Bindungsspezifität: AA 154-227

Spezifität: Specific for all four Rab 3 isoforms a, b, c, d.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant human Rab 3c (aa 154-227).

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC: 1 : 200 up to 1 : 500

Kommentare:

This antibody has been affinity purified with the complete antigen (aa 154 - 227) and recognizes all isoforms, due to the homology of Rab 3 proteins.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Referenzen für anti-RAB3A Antikörper (ABIN1742210)

Romanov, Alpár, Zhang, Zeisel, Calas, Landry, Fuszard, Shirran, Schnell, Dobolyi, Oláh, Spence, Mulder, Martens, Palkovits, Uhlen, Sitte, Botting, Wagner, Linnarsson, Hökfelt, Harkany: "A secretagogin locus of the mammalian hypothalamus controls stress hormone release." in: The EMBO journal, Vol. 34, Issue 1, pp. 36-54, (2015) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

de Wit, Walter, Milosevic, Gulyás-Kovács, Riedel, Sørensen, Verhage: "Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes." in: Cell, Vol. 138, Issue 5, pp. 935-46, (2009) (PubMed).

Details zu RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Andere Bezeichnung: Rab 3

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport