Synaptogyrin 1 Antikörper (AA 170-234)

Produktnummer ABIN1742188

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Synaptogyrin 1 in WB, ELISA, IHC, ICC und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Hamster und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Synaptogyrin 1 Antikörper (AA 170-234) (ABIN1742188)

Target: Synaptogyrin 1 (SYNGR1)

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Synaptogyrin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 80-1

Produktdetails anti-Synaptogyrin 1 Antikörper

Bindungsspezifität: AA 170-234

Spezifität: Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin.

Aufreinigung: purified IgG

Immunogen: Recombinant rat synaptogyrin 1 (aa 170-234).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: 1 : 100

Kommentare:

IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742189 as detector antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-Synaptogyrin 1 Antikörper (ABIN1742188)

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Stenius, Janz, Südhof, Jahn: "Structure of synaptogyrin (p29) defines novel synaptic vesicle protein." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1801-9, (1996) (PubMed).

Details zu Synaptogyrin 1

Target: Synaptogyrin 1 (SYNGR1)

Andere Bezeichnung: Synaptogyrin 1

Hintergrund: Synonyms: p29

Pathways: Regulation of long-term Neuronal Synaptic Plasticity