SHANK3 Antikörper (AA 538-626) (PerCP)
Kurzübersicht für SHANK3 Antikörper (AA 538-626) (PerCP) (ABIN1741266)
Target
Alle SHANK3 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 538-626
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Spezifität
- Detects ~190 kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
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Kreuzreaktivität
- Human, Maus, Ratte
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Aufreinigung
- Protein G Purified
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Immunogen
- Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
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Isotyp
- IgG2a
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Applikationshinweise
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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Kommentare
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1 μg/ml of ABIN1741266 was sufficient for detection of SHANK1/SHANK3 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Conjugated antibodies should be stored at 4°C
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- SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
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Andere Bezeichnung
- SHANK3
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Hintergrund
- SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.
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Gen-ID
- 59312
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UniProt
- Q9JLU4
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Pathways
- Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity
Target
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