MFN2 Antikörper (AA 370-600) (Atto 390)

Produktnummer ABIN1741234

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MFN2 in WB, IHC, ICC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MFN2 Antikörper (AA 370-600) (Atto 390) (ABIN1741234)

Target: MFN2 (Mitofusin 2 (MFN2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MFN2 Antikörper ist konjugiert mit Atto 390

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: S153-5

Produktdetails anti-MFN2 Antikörper (Atto 390)

Bindungsspezifität: AA 370-600

Spezifität: Detects ~90 kDa. No cross-reactivity against Mitofusin-1.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN1741234 was sufficient for detection of Mitofusin-2 in 20 μg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Andere Bezeichnung: Mitofusin 2

Hintergrund: Mitofusin-2 (Mfn2) is a membrane protein of the mitochondria and is important in mitochondrial fusion in mammalian cells. Mfn2 activity has also been implicated in mitochondrial metabolism and the loss of Mfn2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. Mfn2 has been associated with maintaining the membrane potential of the mitochondria. Mfn2 has been shown to hinder mitochondrial antiviral signaling by inhibiting production of type 1 interferons. Mfn2 is a p53-inducible target and involved in cell proliferation, apoptosis promotion and tumor suppression.

Gen-ID: 170731

NCBI Accession: NP_573464

UniProt: Q80U63

Pathways: Skeletal Muscle Fiber Development