DNMT1 Antikörper (AA 637-650)

Produktnummer ABIN1721507

Der Maus Monoklonal Anti-DNMT1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, DNMT1 in Proben von Human, Maus und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für DNMT1 Antikörper (AA 637-650) (ABIN1721507)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reaktivität: Human, Maus, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNMT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 4G11-C7

Produktdetails anti-DNMT1 Antikörper

Bindungsspezifität: AA 637-650

Spezifität: Reacts with human, mouse and zebrafish DNMT1.

Aufreinigung: Purified (protein G)

Immunogen: Synthetic peptide corresponding to aa 637-650 of human DNMT1 (accession no. NP_001370).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(2 μg/mL)

Positive control: ES and H1299 cell lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS (2 mg/mL), pH 7.4.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute in PBS or medium which is identical to that used in the assay system.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one year at -20°C. Avoid repeated freeze-thaw cycles.

Details zu DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Andere Bezeichnung: DNMT1

Hintergrund: Methylation of DNA at cytosine residues is important in regulation of gene expression and genomic imprinting. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked to development of cancer. Thus far three families of mammalian DNA methyltransferase genes have been identified : DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.

DNA (cytosine-5)-methyltransferase 1 CXXC-type zinc finger protein 9, CXXC9 DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI Gene name : DNMT1

Gen-ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion