CD81 Antikörper

Produktnummer ABIN1720914

Dieses Anti-CD81-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD81 in FACS und cELISA. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für CD81 Antikörper (ABIN1720914)

Target: CD81

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD81 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Klon: QV-6A8-S3

Produktdetails anti-CD81 Antikörper

Spezifität: Anti-human CD81 (TAPA-1)

Produktmerkmale: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Aufreinigung: Protein G

Immunogen: genetic immunisation with cDNA encoding human CD81

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Kommentare:

Synonyms: TAPA-1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C

Informationen zur Lagerung: short term: 2 °C - 8 °C, long term: -20 °C

Referenzen für anti-CD81 Antikörper (ABIN1720914)

Fofana, Xiao, Thumann, Turek, Zona, Tawar, Grunert, Thompson, Zeisel, Baumert: "A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission." in: PLoS ONE, Vol. 8, Issue 5, pp. e64221, (2013) (PubMed).

Details zu CD81

Target: CD81

Andere Bezeichnung: CD81

Hintergrund: CD81 (TAPA-1) belongs to the transmembrane 4 superfamily (tetraspanin family). CD81 is a widely expressed cell-surface protein that is characterized by the presence of four transmembranic domains, short N and C termini, a small extracellular loop (SEL) and a large extra-cellular loop (LEL). It is expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin and plays a role in the regulation of cell development, cell-growth and signal transduction. CD81 plays a critical role in Hepatitis C Infection and is involved in HCV entry due to its ability to interact with virus' E1/E2 glycoproteins. CD81 is an essential HCV host factor as silencing of CD81 expression by CD81-specific monoclonal antibodies in hepatoma cells inhibits HCV entry.

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C