CpxA (N-Term) Antikörper

Produktnummer ABIN1720869

Der Kaninchen Polyklonal anti-CpxA Antikörper (ABIN1720869) detektiert spezifisch CpxA in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Yersinia pseudotuberculosis.

Kurzübersicht für CpxA (N-Term) Antikörper (ABIN1720869)

Target: CpxA

Reaktivität: Yersinia pseudotuberculosis

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Produktdetails

Bindungsspezifität: N-Term

Verwendungszweck: Anti-CpxA | Conjugative plasmid expression

Kreuzreaktivität (Details): Yersinia pseudotuberculosis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Aufreinigung: Serum

Immunogen: Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag

Anwendungsinformationen

Applikationshinweise: 1 : 2000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: CpxA

Hintergrund:

Background: CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.

Additional information: The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1,5), To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa, CpxA could be routinely detected from a 20 μL of sample that was derived from 1 mL of pelleted bacteria resuspended in 200 μL of 1x loading buffer, Re-use of diluted working strength antibody is not recommended

Molekulargewicht: 51.8 | 52-53 kDa