CpxA (N-Term) Antikörper

Produktnummer ABIN1720869

Produktdetails

Target: CpxA

Bindungsspezifität: N-Term

Reaktivität: Yersinia pseudotuberculosis

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity are currently known

Homologie: Yersinia pestis, Yersinia enterocolica and related species

Aufreinigung: serum

Immunogen: Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII, B1JQV1, NCBI annotated locus tag YPK_4133.

Anwendungsinformationen

Applikationshinweise: 1 : 2000 with standard ECL (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Handhabung: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Antigendetails

Target: CpxA

Hintergrund: CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.

Molekulargewicht: expected: 51.8 kDa, appearent: 52-53 kDa