Actin Antikörper

Produktnummer ABIN1720863

Dieses Anti-Actin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Actin in WB und IF. Geeignet für Gerste, Arabidopsis thaliana, Soybean und Mais (Zea mays). Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Actin Antikörper (ABIN1720863)

Target: Actin

Reaktivität: Gerste, Arabidopsis thaliana, Soybean, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-Actin Antikörper

Spezifität: TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520

Kreuzreaktivität (Details): Not reactive in: Chlamydomonas reinhardtii (too high background for this species)

Homologie: Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba

Aufreinigung: serum

Immunogen: ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520

Anwendungsinformationen

Applikationshinweise: 1 : 2500 with standard ECL (WB), 1: 250 (IF)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Handhabung: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-Actin Antikörper (ABIN1720863)

Zheng, Fang, Pan, Sun, Deng, Grosser, Guo: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).

Details zu Actin

Target: Actin

Andere Bezeichnung: Actin (ACT)

Hintergrund: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Molekulargewicht: expected: 41.6 kDa, appearent: 45 kDa

UniProt: P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497