PRKCSH Antikörper (AA 101-200)

Produktnummer ABIN1715023

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch PRKCSH in WB, ELISA, ICC, IF (p), IF (cc), IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PRKCSH Antikörper (AA 101-200) (ABIN1715023)

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKCSH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PRKCSH Antikörper

Bindungsspezifität: AA 101-200

Homologie: Human,Mouse,Rat,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glucosidase 2 subunit beta

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Andere Bezeichnung: Glucosidase 2 subunit beta

Hintergrund:

Synonyms: 80K-H protein, AGE-binding receptor 2, AGE-R2, G19P1, GLU2B_HUMAN, Glucosidase 2 subunit beta, Glucosidase II beta subunit, Glucosidase II subunit beta, Hepatocystin, PCLD, PKCSH, PLD1, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain, Protein kinase C substrate 80 Kda protein, Protein kinase C substrate 80K-H, Protein kinase C substrate, 80 Kda protein.

Background: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains a and b subunits (Glucosidase IIa and Glucosidase IIb) which form a defined heterodimeric complex. Glucosidase IIa is the catalyitc core of the enzyme and can function independently of the b subunit. The sequence of Glucosidase IIb encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C-terminus. The phosphorylated form of Glucosidase IIb is localized in the plasma membrane and is highly expressed in FGF-stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIb was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIb retains the complete complex in the ER.

Gen-ID: 5589

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process