PRKCSH Antikörper (AA 101-200)

Produktnummer ABIN1715023

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PRKCSH in WB, ELISA, IF (cc), IF (p), ICC, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PRKCSH Antikörper (AA 101-200) (ABIN1715023)

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKCSH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PRKCSH Antikörper

Bindungsspezifität: AA 101-200

Homologie: Human,Mouse,Rat,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glucosidase 2 subunit beta

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Andere Bezeichnung: Glucosidase 2 subunit beta

Hintergrund:

Synonyms: 80K-H protein, AGE-binding receptor 2, AGE-R2, G19P1, GLU2B_HUMAN, Glucosidase 2 subunit beta, Glucosidase II beta subunit, Glucosidase II subunit beta, Hepatocystin, PCLD, PKCSH, PLD1, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain, Protein kinase C substrate 80 Kda protein, Protein kinase C substrate 80K-H, Protein kinase C substrate, 80 Kda protein.

Background: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains a and b subunits (Glucosidase IIa and Glucosidase IIb) which form a defined heterodimeric complex. Glucosidase IIa is the catalyitc core of the enzyme and can function independently of the b subunit. The sequence of Glucosidase IIb encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C-terminus. The phosphorylated form of Glucosidase IIb is localized in the plasma membrane and is highly expressed in FGF-stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIb was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIb retains the complete complex in the ER.

Gen-ID: 5589

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process