ORC1 Antikörper

Produktnummer ABIN1713877

Produktdetails anti-ORC1 Antikörper

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ORC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ORC1L/ORC1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu ORC1

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Andere Bezeichnung: ORC1L (ORC1L Produkte)

Synonyme: CG10667 antikoerper, DmORC1 antikoerper, DmOrc1 antikoerper, DmOrc1p antikoerper, Dmel\\CG10667 antikoerper, ORC antikoerper, ORC1 antikoerper, dORC1 antikoerper, orc1 antikoerper, rDmORC antikoerper, ORC1L antikoerper, HSORC1 antikoerper, PARC1 antikoerper, orc1l antikoerper, xorc1 antikoerper, AA545195 antikoerper, MmORC1 antikoerper, Orc1l antikoerper, Origin recognition complex subunit 1 antikoerper, origin recognition complex subunit 1 antikoerper, origin recognition complex subunit 1 L homeolog antikoerper, origin recognition complex, subunit 1 antikoerper, Orc1 antikoerper, ORC1 antikoerper, orc1.L antikoerper

Hintergrund:

Synonyms: HSORC1, MmORC1, orc1, ORC1_HUMAN, ORC1L, Origin Recognition Complex 1, Origin recognition complex subunit 1 yeast homolog like, Origin recognition complex subunit 1, Origin recognition complex subunit 1 homolog, Origin recognition complex subunit 1 like S. cerevisia, Origin recognition complex subunit 1 like, Origin recognition complex subunit 1 S. cerevisiae homolog like, Origin recognition complex, subunit 1 like yeast, PARC1, Replication control protein 1.

Background: The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation (1). Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins (1). ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors (1). Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis (2). ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle, however, only ORC2 remains stably bound to chromatin (3,4). ORC4 and ORC6 are also expressed constantly throughout the cell cycle (5,6). ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble (7,8). The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells (8).

Gen-ID: 4998

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA