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CD34 Antikörper (AA 210-260)

Name: CD34 Antikörper (AA 210-260)
SKU: ABIN1713693
Price: 357.7 EUR
Availability: InStock

Dieses Anti-CD34-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD34 in FACS, WB, IHC (p) und IF (p). Geeignet für Human, Ratte, Maus und Schwein. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (CD34 Antikörper (AA 210-260))

FACS

Mouse heart lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (CD34 Antikörper (AA 210-260))

Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-CD34 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD34 Antikörper (AA 210-260))

IHC (p)

3 Abbildungen

(2 Referenzen)

Produktnummer ABIN1713693

Datenblatt (PDF)

Kurzübersicht für CD34 Antikörper (AA 210-260) (ABIN1713693)

Target

Alle CD34 Antikörper anzeigen

CD34

Reaktivität

Human, Ratte, Maus, Schwein

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser CD34 Antikörper ist unkonjugiert

Applikation

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

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Bindungsspezifität
22

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5

3

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AA 210-260

Kreuzreaktivität

Human, Maus, Schwein, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD34

Isotyp

IgG
anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 Reaktivität: Human, Ratte, Maus FACS, WB, IHC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) (AA 201-300) antibody
CD34 Reaktivität: Human, Ratte, Maus, Kaninchen FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) (AA 301-385) antibody
CD34 Reaktivität: Human, Ratte, Maus, Kaninchen, Huhn, Ziege FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 Reaktivität: Human, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), Func Wirt: Maus Monoclonal QBEnd-10 unconjugated

anti-CD34 (CD34) (AA 36-287) antibody
CD34 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody (PE)
CD34 Reaktivität: Human FACS, ICC Wirt: Maus Monoclonal 4H11[APG] PE

anti-CD34 (CD34) (C-Term) antibody
CD34 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 Reaktivität: Human, Ratte FACS, IHC, StM Wirt: Kaninchen Monoclonal HPCA1-2598R unconjugated Recombinant Antibody

anti-CD34 (CD34) (AA 151-385) antibody
CD34 Reaktivität: Human, Ratte WB, IHC, IF, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 Reaktivität: Human FACS, WB, IHC (p), ICC Wirt: Maus Monoclonal 4H11[APG] unconjugated

Applikationshinweise

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Lee, Bae, Park, Jang, Shim, Lim, Park, Sim, Jeong: "Comparison of dextran-based sirolimus-eluting stents and PLA-based sirolimus-eluting stents in vitro and in vivo." in: Journal of biomedical materials research. Part A, Vol. 105, Issue 1, pp. 301-310, (2016) (PubMed).

Cui, Zheng, Gong, Wen, Ma, Zhou, Zhang: "Effects of Bu-Shen-An-Tai recipe and its two components on endometrial morphology during peri-implantation in superovulated mice." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 5, pp. 768-74, (2014) (PubMed).
Target

CD34

Andere Bezeichnung

CD34

Hintergrund

Synonyms: Hematopoietic progenitor cell antigen CD34, CD34
Background: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Gen-ID

947

UniProt

P28906