IFI30 Antikörper (AA 181-250) (HRP)

Produktnummer ABIN1711361

Der Kaninchen Polyklonal Anti-IFI30-Antikörper wurde für ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, IFI30 in Proben von Human zu detektieren.

Kurzübersicht für IFI30 Antikörper (AA 181-250) (HRP) (ABIN1711361)

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFI30 Antikörper ist konjugiert mit HRP

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-IFI30 Antikörper (HRP)

Bindungsspezifität: AA 181-250

Homologie: Human,Mouse,Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IFI30

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu IFI30

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Andere Bezeichnung: I30

Hintergrund:

Synonyms: Gamma interferon inducible lysosomal thiol reductase, Gamma interferon inducible protein IP 30, GILT, I 30, interferon gamma inducible protein 30, Interferon gamma inducible protein 30 preproprotein, IP30, Lysosomal thiol reductase, Lysosomal thiol reductase, gamma-interferon-inducible.

Background: Proteins internalized into the endocytic pathway are usually degraded. Efficient proteolysis requires denaturation, induced by acidic conditions within lysosomes, and reduction of inter- and intrachain disulfide bonds. Cytosolic reduction is mediated enzymatically by thioredoxin. In the endocytic pathway, reduction of protein disulfide bonds is important for the generation of MHC class II-peptide complexes. This process is catalyzed by a gamma-interferon-inducible thiol reductase (GILT). GILT is synthesized as a precursor, and following delivery to MHC class II-containing compartments (MIICs), is processed to the mature form via cleavage of amino- and carboxy-terminal propeptides. A lysosomal thiol reductase, GILT, is optimally active at low pH and capable of catalyzing disulfide bond reduction both in vivo and in vitro. GILT is expressed constitutively in antigen-presenting cells and is induced by g-interferon in other cell types, suggesting a potentially important role in antigen processing. Additionally, T cell recognition of select exogenous and endogenous epitopes is dependent on tumor cell expression of GILT. The absence of GILT in melanomas alters antigen processing and the hierarchy of immunodominant epitope presentation.

Gen-ID: 10437

UniProt: P13284