NGEF Antikörper (pTyr179) (Cy7)

Produktnummer ABIN1707872

Der Kaninchen Polyklonal Anti-NGEF-Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, NGEF in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NGEF Antikörper (pTyr179) (Cy7) (ABIN1707872)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NGEF Antikörper ist konjugiert mit Cy7

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-NGEF Antikörper (Cy7)

Bindungsspezifität: pTyr179

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ephexin-1 around the phosphorylation site of Tyr179

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu NGEF

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Andere Bezeichnung: Ephexin-1

Hintergrund:

Synonyms: Ephexin-1 phospho Y179, p-Ephexin-1 phospho Y179, ARHGEF27, Eph-interacting exchange protein, EPHEXIN, Ephexin 1, Neuronal guanine nucleotide exchange factor, Ngef, NGEF_HUMAN.

Background: EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.

Gen-ID: 25791

Pathways: Neurotrophin Signalübertragung