ZBTB7A Antikörper (AA 321-420) (Cy5)

Produktnummer ABIN1704343

Produktdetails anti-ZBTB7A Antikörper (Cy5)

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Bindungsspezifität: AA 321-420

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZBTB7A Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Pokemon

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ZBTB7A

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Andere Bezeichnung: Pokemon/ZBTB7 (ZBTB7A Produkte)

Synonyme: ZBTB7A antikoerper, FBI-1 antikoerper, FBI1 antikoerper, LRF antikoerper, ZBTB7 antikoerper, ZNF857A antikoerper, pokemon antikoerper, 9030619K07Rik antikoerper, 9130006G12Rik antikoerper, AI452336 antikoerper, Lrf antikoerper, Pokemon antikoerper, Zbtb7 antikoerper, OCZF antikoerper, zinc finger and BTB domain containing 7A antikoerper, zinc finger and BTB domain containing 7C antikoerper, zinc finger and BTB domain containing 7a antikoerper, ZBTB7A antikoerper, ZBTB7C antikoerper, Zbtb7a antikoerper

Hintergrund:

Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.

Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

Gen-ID: 51341