ARFGEF1 Antikörper (AA 1-200) (Cy5)

Produktnummer ABIN1704235

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ARFGEF1 in WB, IF (cc) und IF (p). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für ARFGEF1 Antikörper (AA 1-200) (Cy5) (ABIN1704235)

Target: ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARFGEF1 Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-ARFGEF1 Antikörper (Cy5)

Bindungsspezifität: AA 1-200

Kreuzreaktivität: Maus

Homologie: Human,Rat,Dog,Cow,Sheep,Pig,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu ARFGEF1

Target: ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))

Andere Bezeichnung: BIG1/ARFGEF1

Hintergrund:

Synonyms: ADPribosylation factor guanine nucleotide exchange factor 1brefeldin A inhibited, ARFGEF1, ARFGEP1, Brefeldin A inhibited GEP 1, Brefeldin A inhibited guanine nucleotide exchange protein 1, p200 ARF GEP1, p200 ARF guanine nucleotide exchange factor, BIG1_HUMAN.

Background: Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.

Gen-ID: 10565

Pathways: Regulation of Actin Filament Polymerization, Regulation of Carbohydrate Metabolic Process