ZBTB7A Antikörper (AA 321-420) (Biotin)

Produktnummer ABIN1700719

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ZBTB7A in WB, ELISA, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ZBTB7A Antikörper (AA 321-420) (Biotin) (ABIN1700719)

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZBTB7A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ZBTB7A Antikörper (Biotin)

Bindungsspezifität: AA 321-420

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Pokemon

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu ZBTB7A

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Andere Bezeichnung: Pokemon/ZBTB7

Hintergrund:

Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.

Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

Gen-ID: 51341