CD200 Antikörper (APC)

Produktnummer ABIN1690745

Der Maus Monoklonal Anti-CD200-Antikörper wurde für FACS validiert. Er ist geeignet, CD200 in Proben von Human zu detektieren. Es sind 7+ Publikationen verfügbar.

Kurzübersicht für CD200 Antikörper (APC) (ABIN1690745)

Target: CD200

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD200 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: OX-104

Produktdetails anti-CD200 Antikörper (APC)

Verwendungszweck: Anti-Hu CD200 APC

Spezifität: The mouse monoclonal antibody OX-104 recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: recombinant human CD200

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD200 Antikörper (APC) (ABIN1690745)

Colmont, Benketah, Reed, Hawk, Telford, Ohyama, Udey, Yee, Vogel, Patel: "CD200-expressing human basal cell carcinoma cells initiate tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 4, pp. 1434-9, (2013) (PubMed).

Li, Zhao, Tong, Qian, Ren, Zhang, Ding, Chen, Wang, Zhang, Zeng, Zhang, Tang, Zhang, Ba, He, Cao, Lipsky: "Aberrant CD200/CD200R1 expression and function in systemic lupus erythematosus contributes to abnormal T-cell responsiveness and dendritic cell activity." in: Arthritis research & therapy, Vol. 14, Issue 3, pp. R123, (2012) (PubMed).

Garza, Yang, Zhao, Blatt, Lee, He, Stanton, Carrasco, Spiegel, Tobias, Cotsarelis: "Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells." in: The Journal of clinical investigation, Vol. 121, Issue 2, pp. 613-22, (2011) (PubMed).

Meuth, Simon, Grimm, Melzer, Herrmann, Spitzer, Landgraf, Wiendl: "CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing." in: Journal of neuroimmunology, Vol. 194, Issue 1-2, pp. 62-9, (2008) (PubMed).

Sopp, Werling, Baldwin: "Cross-reactivity of mAbs to human CD antigens with cells from cattle." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 106-14, (2007) (PubMed).

Shiratori, Yamaguchi, Suzukawa, Yamamoto, Lanier, Saito, Arase: "Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 7, pp. 4441-9, (2005) (PubMed).

Foster-Cuevas, Wright, Puklavec, Brown, Barclay: "Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor." in: Journal of virology, Vol. 78, Issue 14, pp. 7667-76, (2004) (PubMed).

Details zu CD200

Target: CD200

Andere Bezeichnung: CD200

Hintergrund: CD200 Molecule,CD200 (also known as OX2 or MRC) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization.,MRC, MOX, OX-2

Gen-ID: 4345

UniProt: P41217