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pan Keratin Antikörper

panKRT Reaktivität: Human IHC (p), IHC (fro) Wirt: Maus Monoclonal B311-1 unconjugated

Produktnummer ABIN966802

Datenblatt (PDF)

Target Alle pan Keratin (panKRT) Antikörper anzeigen

pan Keratin (panKRT)
Reaktivität
82

58

39

34

22

20

20

19

19

15

15

14

10

6

1

1

1
Human
Wirt
54

34

1
Maus
Passende Sekundärantikörper
Klonalität
57

32
Monoklonal
Konjugat
33

6

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser pan Keratin Antikörper ist unkonjugiert
Applikation
55

54

49

24

19

16

14

14

7

7

6

5

3

2
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität

This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.

Aufreinigung

Concentrated.

Immunogen

Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

Klon

B311-1

Isotyp

IgG1 kappa

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panKRT Reaktivität: Human, Ratte IHC (p), ICC, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

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panKRT Reaktivität: Säugetier FACS Wirt: Maus Monoclonal C-11 PE

anti-pan Keratin (panKRT) antibody (APC)
panKRT Reaktivität: Säugetier FACS Wirt: Maus Monoclonal C-11 APC

anti-pan Keratin (panKRT) antibody (Biotin)
panKRT Reaktivität: Säugetier FACS, WB, IHC (p), ICC, IP Wirt: Maus Monoclonal C-11 Biotin

anti-pan Keratin (panKRT) antibody
panKRT Reaktivität: Human IHC Wirt: Kaninchen Monoclonal MSVA-000R unconjugated Recombinant Antibody

Applikationshinweise

IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.

Kommentare

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.35 mg/ml.

Buffer

20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C
Target

pan Keratin (panKRT)

Andere Bezeichnung

Pan Cytokeratin, Pan CK-II (panKRT Produkte)

Synonyme

xk70a antikoerper, keratin antikoerper, keratin S homeolog antikoerper, LOC431321 antikoerper, krt.S antikoerper

Molekulargewicht

45, 52.5 ,54, 56, 56.5, 58, & 59 kDa