pan Keratin Antikörper

Produktnummer ABIN966802

Dieses Maus Monoklonal-Antikörper erkennt spezifisch pan Keratin in IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für pan Keratin Antikörper (ABIN966802)

Target: pan Keratin (panKRT)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B311-1

Produktdetails anti-pan Keratin Antikörper

Spezifität: This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.

Aufreinigung: Concentrated.

Immunogen: Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.

Kommentare:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.35 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Pan Cytokeratin, Pan CK-II

Molekulargewicht: 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa