TCP1 alpha/CCTA Antikörper (C-Term)

Produktnummer ABIN1686651

Dieses Anti-TCP1 alpha/CCTA-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von TCP1 alpha/CCTA in WB, IF, FACS, IP und ICC. Geeignet für Maus.

Kurzübersicht für TCP1 alpha/CCTA Antikörper (C-Term) (ABIN1686651)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TCP1 alpha/CCTA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 91a

Produktdetails anti-TCP1 alpha/CCTA Antikörper

Bindungsspezifität: C-Term

Spezifität: Detects ~60 & ~92 kDa. Cross reactivity with human HSP60 in WB. Reacts weakly with S. cerevisiae, consistent with the epitope being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recog TCP1 of P. sativum, and the sequence of A. thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.

Kreuzreaktivität: C. elegans, Rind (Kuh), Hund, Drosophila melanogaster, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Saccharomyces cerevisiae

Aufreinigung: Protein G Purified

Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN1686651 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Andere Bezeichnung: TCP1 alpha

Hintergrund: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.

Gen-ID: 21454

NCBI Accession: NP_038714

UniProt: P11983