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SYT7 Antikörper (AA 150-239)

This anti-SYT7 antibody (ABIN1686633) is a Mouse Monoclonal antibody detecting SYT7 in WB, IHC, ICC, IF. Suitable for Mouse.
Produktnummer ABIN1686633

Quick Overview for SYT7 Antikörper (AA 150-239) (ABIN1686633)

Target

Alle SYT7 Antikörper anzeigen
SYT7 (Synaptotagmin VII (SYT7))

Reaktivität

  • 27
  • 25
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Maus

Wirt

  • 25
  • 17
  • 1
Maus

Klonalität

  • 26
  • 17
Monoklonal

Konjugat

  • 21
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser SYT7 Antikörper ist unkonjugiert

Applikation

  • 35
  • 17
  • 13
  • 10
  • 9
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon

S275-14
  • Bindungsspezifität

    • 10
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 150-239

    Spezifität

    Detects ~45 kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.

    Kreuzreaktivität

    Human, Maus, Ratte

    Aufreinigung

    Protein G Purified

    Immunogen

    Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7

    Isotyp

    IgG2b
  • Applikationshinweise

    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    1 μg/ml of ABIN1686633 was sufficient for detection of Synaptotagmin-7 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    -20°C
  • Target

    SYT7 (Synaptotagmin VII (SYT7))

    Andere Bezeichnung

    Synaptotagmin 7

    Hintergrund

    Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2.

    Gen-ID

    54525

    NCBI Accession

    NP_061271

    UniProt

    Q9R0N7

    Pathways

    Synaptic Vesicle Exocytosis
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