SYT7 Antikörper (AA 150-239)
Kurzübersicht für SYT7 Antikörper (AA 150-239) (ABIN1686633)
Target
Alle SYT7 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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                                            Bindungsspezifität
- AA 150-239
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                                            Spezifität
- Detects ~45 kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
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                                            Kreuzreaktivität
- Human, Maus, Ratte
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                                            Aufreinigung
- Protein G Purified
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                                            Immunogen
- Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
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                                            Isotyp
- IgG2b
 
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                                            Applikationshinweise
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                        - WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
 
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                                            Kommentare
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                        1 μg/ml of ABIN1686633 was sufficient for detection of Synaptotagmin-7 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. 
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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                                            Format
- Liquid
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                                            Konzentration
- 1 mg/mL
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                                            Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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                                            Konservierungsmittel
- Sodium azide
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                                            Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            Lagerung
- -20 °C
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                                            Informationen zur Lagerung
- -20°C
 
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    - SYT7 (Synaptotagmin VII (SYT7))
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                                            Andere Bezeichnung
- Synaptotagmin 7
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                                            Hintergrund
- Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2.
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                                            Gen-ID
- 54525
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                                            NCBI Accession
- NP_061271
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                                            UniProt
- Q9R0N7
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                                            Pathways
- Synaptic Vesicle Exocytosis
 Target
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