Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Thylakoid Membranes in WB. Es zeigt Reaktivität gegenüber Proben von Arabidopsis, Pisum sativum und Spinach.
Kurzübersicht für Thylakoid Membranes Antikörper (ABIN165370)
Target
Thylakoid Membranes
Reaktivität
Arabidopsis, Pisum sativum, Spinach
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
Spezifität
Higher plants, cyanobacteria
Immunogen
extracted thylakoid membrane proteins from spinach chloroplasts. Intact chloroplasts from spinach were isolated by isopycnic Percoll gradient centrifugation. Thylakoids were separated from stromal proteins after osmotic shock of chloroplasts. Thylakoids were washed in Tricine/Sucrose/MgCl 2 -containing buffer and broken twice by a French Press. The thylakoid membrane proteins were separated from lumenal proteins by ultra centrifugation and following washing steps of thylakoid membranes.