Der Kaninchen Polyklonal anti-Thylakoid Proteins Antikörper (ABIN165369) detektiert spezifisch Thylakoid Proteins in WB.
Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis, Pisum sativum und Spinach.
Kurzübersicht für Thylakoid Proteins Antikörper (ABIN165369)
Target
Thylakoid Proteins
Reaktivität
Arabidopsis, Pisum sativum, Spinach
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Western Blotting (WB)
Spezifität
Higher plants, Synechocystis . Other photosynthetic organisms were not tested
Immunogen
extracted thylakoid proteins from spinach. Intact chloroplasts from spinach were isolated by isopycnic Percoll gradient centrifugation. Thylakoids were separated from stromal proteins after osmotic shock of chloroplasts. Thylakoids were washed in Tricine/Sucrose/MgCl 2 -containing buffer and used for rabbit immunisation. The thylakoid protein fraction contained thylakoid membrane and lumenal proteins.
Isotyp
IgG
Applikationshinweise
Western blot (ECL - 1 : 3.000 for higher plant proteins and 1: 1.000 for Synechocystis proteins)