HDAC5 Antikörper (pSer661)

Produktnummer ABIN1607888

Hersteller Produkt- Nr.: 600-401-j21

Der Kaninchen Polyklonal Anti-HDAC5-Antikörper wurde für WB, IHC, ELISA, DB und FM validiert. Er ist geeignet, HDAC5 in Proben von Human und Maus zu detektieren.

Kurzübersicht für HDAC5 Antikörper (pSer661) (ABIN1607888)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HDAC5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-HDAC5 Antikörper

Bindungsspezifität: pSer661

Hersteller Produkt- Nr.: 600-401-j21

Hersteller: Rockland

Verwendungszweck: HDAC5 (internal) Antibody

Kreuzreaktivität (Details): This antibody is specific towards HDAC5.

Produktmerkmale: Synonyms: rabbit anti-HDAC-5 antibody, HDAC5, HDAC 5, HD 5 antibody, histone deacetylase 5 antibody, Histone deacetylase-5, HD5 antibody, NY-CO-9

Aufreinigung: Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-HDAC5 antibody has been tested in ELISA, dot blot, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.2 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.12 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 6 months

Details zu HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Andere Bezeichnung: HDAC5

Hintergrund: Background: HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. Anti-HDAC5 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.

Gen-ID: 10014

NCBI Accession: NP_005465

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process