ampC Antikörper (Biotin)

Produktnummer ABIN1607809

Hersteller Produkt- Nr.: 200-4675-0100

Der Kaninchen Polyklonal Anti-ampC-Antikörper wurde für WB, IP und DB validiert. Er ist geeignet, ampC in Proben von Enterobacter cloacae zu detektieren.

Kurzübersicht für ampC Antikörper (Biotin) (ABIN1607809)

Target: ampC (beta-Lactamase (ampC))

Reaktivität: Enterobacter cloacae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ampC Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Produktdetails anti-ampC Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-4675-0100

Hersteller: Rockland

Verwendungszweck: Penicillinase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].

Produktmerkmale: Synonyms: rabbit anti-Penicillinase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase

Aufreinigung: Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Penicillinase [Enterobacter cloacae]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Penicillinase Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu ampC

Target: ampC (beta-Lactamase (ampC))

Andere Bezeichnung: ampC

Hintergrund: Background: Anti-Penicillinase antibody recognizes the protein penicillinase, a beta lactamase specific to penicillins. Penicillinase hydrolyzes the beta-lactam ring of penicillins destroying their antibacterial potency. Thus, penicillinase is often found in antibiotic-resistant bacteria.

Gen-ID: 9125732

UniProt: P05364