PCK1 Antikörper (Biotin)

Produktnummer ABIN1607799

Produktdetails anti-PCK1 Antikörper (Biotin)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reaktivität: Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCK1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Phospho-enol-Pyruvate-Carboxylase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Phospho Enol Pyruvate Carboxylase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate-Carboxylase [Maize].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Phospho Enol Pyruvate Carboxylase antibody has been assayed against 1.0 µg of Phospho-enol-Pyruvate-Carboxylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:12.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Andere Bezeichnung: Phospho Enol Pyruvate Carboxylase (PCK1 Produkte)

Synonyme: PEPCK-C antikoerper, PEPCK1 antikoerper, PEPCKC antikoerper, PEPCK antikoerper, PEPCK-M antikoerper, PEPCK2 antikoerper, 1810010O14Rik antikoerper, 9130022B02Rik antikoerper, cb856 antikoerper, cb924 antikoerper, fj93h11 antikoerper, zgc:63869 antikoerper, wu:fc51c05 antikoerper, wu:fj93h11 antikoerper, pepck-c antikoerper, pepck1 antikoerper, pepckc antikoerper, PCK1 antikoerper, PPCK1 antikoerper, AI265463 antikoerper, Pck-1 antikoerper, GTP antikoerper, PCK antikoerper, Pepck antikoerper, RATPEPCK antikoerper, Ppc1C antikoerper, PHOSPHOENOLPYRUVATE CARBOXYKINASE antikoerper, T28I19.150 antikoerper, T28I19_150 antikoerper, phosphoenolpyruvate carboxykinase 1 antikoerper, 143299_at antikoerper, CG10924 antikoerper, CG17725 antikoerper, Dmel\\CG17725 antikoerper, Dromel_CG17725_FBtr0086701_pepck_mORF antikoerper, dPEPCK antikoerper, pepck antikoerper, PEPC antikoerper, PEPCase antikoerper, ppc antikoerper, phosphoenolpyruvate carboxykinase 1 antikoerper, phosphoenolpyruvate carboxykinase 2, mitochondrial antikoerper, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antikoerper, phosphoenolpyruvate carboxykinase 1 (soluble) antikoerper, phosphoenolpyruvate carboxykinase 1 S homeolog antikoerper, phosphoenolpyruvate carboxykinase, cytosolic [GTP] antikoerper, phosphoenolpyruvate carboxykinase 1, cytosolic antikoerper, phosphoenolpyruvate carboxylase 7 antikoerper, Phosphoenolpyruvate carboxykinase antikoerper, phosphoenolpyruvate carboxylase antikoerper, PCK1 antikoerper, PCK2 antikoerper, Pck2 antikoerper, pck1 antikoerper, pck1.S antikoerper, LOC100634531 antikoerper, Pck1 antikoerper, pep7 antikoerper, Pepck antikoerper, LOC107777405 antikoerper

Hintergrund: Anti-Phospho Enol Pyruvate Carboxylase antibody detects PEP. Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate. This reaction is used for carbon fixation in so-called Synonyms: PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody

Gen-ID: 542372

UniProt: B8XPZ2

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis