Mutarotase Antikörper (Biotin)

Produktnummer ABIN1607798

Hersteller Produkt- Nr.: 200-4662s

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, in Proben von Schwein zu detektieren.

Kurzübersicht für Mutarotase Antikörper (Biotin) (ABIN1607798)

Target: Mutarotase

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails

Hersteller Produkt- Nr.: 200-4662s

Hersteller: Rockland

Verwendungszweck: Mutarotase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Mutarotase [Porcine Kidney].

Produktmerkmale: Synonyms: rabbit anti-Mutarotase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Mutarotase Antibody, Aldose 1 epimerase antibody, BLOCK25 protein antibody, Galactose mutarotase antibody, GALM antibody

Aufreinigung: Anti-Mutarotase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Mutarotase [Porcine Kidney]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Mutarotase antibody has been tested in WB and assayed against 1.0 μg of Mutarotase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: Mutarotase

Hintergrund: Background: Anti-Mutarotase antibody recognizes the protein mutarotase. Mutarotase catalyzes the mutarotation of alpha-aldose to the beta-anomer. Its substrates include D-glucose, L-arabinose, D-xylose, D-galactose, maltose, and lactose.

Gen-ID: 399536

UniProt: Q9GKX6