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ADH1 Antikörper (Biotin)

Der Kaninchen Polyklonal Anti-ADH1-Antikörper wurde für WB, IP und ELISA validiert. Er ist geeignet, ADH1 in Proben von Saccharomyces cerevisiae zu detektieren.
Rockland
Produktnummer ABIN1607773
Hersteller Produkt- Nr.: 200-4643-0100

Kurzübersicht für ADH1 Antikörper (Biotin) (ABIN1607773)

Target

Alle ADH1 Antikörper anzeigen
ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reaktivität

  • 9
  • 4
  • 4
  • 2
Saccharomyces cerevisiae

Wirt

  • 16
  • 3
Kaninchen

Klonalität

  • 16
  • 3
Polyklonal

Konjugat

  • 14
  • 3
  • 2
Dieser ADH1 Antikörper ist konjugiert mit Biotin

Applikation

  • 19
  • 14
  • 12
  • 5
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), ELISA
  • Hersteller Produkt- Nr.

    200-4643-0100

    Hersteller

    Rockland

    Verwendungszweck

    Alcohol Dehydrogenase Antibody Biotin Conjugated

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].

    Produktmerkmale

    Synonyms: rabbit anti-Alcohol Dehydrogenase Antibody biotin Conjugation, biotin conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC

    Aufreinigung

    Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Immunogen: Alcohol Dehydrogenase [Yeast]

    Immunogen Type: Native Protein

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: Anti-Alcohol Dehydrogenase Biotin Conjugated Antibody has been tested in ELISA and Western blot. This product is assayed against 1.0 μg of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:2,000 - 1:15,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    10 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Target

    ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

    Andere Bezeichnung

    ADH1

    Hintergrund

    Background: Alcohol Dehydrogenase is part of the zinc-containing alcohol dehydrogenase family. Anti-Alcohol Dehydrogenase catalyzes primary branched alcohols to convert to their aldehyde forms and shows some reactivity with secondary alcohols. Localized in the cytoplasm, the Anti-Alcohol Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cancer research.

    Gen-ID

    2538902, 12643994

    UniProt

    P00330
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