Glucose Oxidase Antikörper (Biotin)

Produktnummer ABIN1607767

Produktdetails anti-Glucose Oxidase Antikörper (Biotin)

Target: Glucose Oxidase (GOD)

Reaktivität: Aspergillus niger

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucose Oxidase Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB)

Kreuzreaktivität (Details): Cross reactivity against Glucose Oxidase from other sources may occur but have not been specifically determined.

Reinheit: Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.niger].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Glucose Oxidase [A.niger]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-GLUCOSE OXIDASE Antibody has been assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product. Anti-GLUCOSE OXIDASE Antibody has been assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product. Specific condtions should be optimized by user.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Glucose Oxidase

Target: Glucose Oxidase (GOD)

Andere Bezeichnung: Glucose Oxidase (GOD Produkte)

Synonyme: LOC406081 antikoerper, GB19418 antikoerper, glucose oxidase antikoerper, LOC406081 antikoerper, AFUA_2G14840 antikoerper, SS1G_10949 antikoerper, AOR_1_952194 antikoerper, AOR_1_786174 antikoerper, AOR_1_358034 antikoerper, CC1G_15386 antikoerper, VDBG_09040 antikoerper, VDBG_06502 antikoerper, SCHCODRAFT_113971 antikoerper

Hintergrund: Glucose Oxidase antibody detect the glucose oxidase protein. The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids (diagnostics), in vegetal raw material, and in the food industry. GOx is a dimeric protein, the 3D structure of which has been elucidated. The active site where glucose binds is in a deep pocket. The enzyme, like many proteins that act outside of cells, is covered with carbohydrate chains. At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6 % β-D-glucopyranose and 36.4 % α-D-glucopyranose, the proportion of linear and furanose form being negligible. Anti-Glucose Oxidase antibody is ideal for investigators involved in glucose energy metabolism research. Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Gen-ID: 2683500

UniProt: P13006