Intestinal Alkaline Phosphatase Antikörper (Biotin)

Produktnummer ABIN1607763

Hersteller Produkt- Nr.: 200-4635-0100

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Intestinal Alkaline Phosphatase in WB und ELISA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für Intestinal Alkaline Phosphatase Antikörper (Biotin) (ABIN1607763)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Intestinal Alkaline Phosphatase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Intestinal Alkaline Phosphatase Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-4635-0100

Hersteller: Rockland

Verwendungszweck: Alkaline Phosphatase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Produktmerkmale: Synonyms: rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Alkaline Phosphatase [Calf Intestine]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Andere Bezeichnung: Calf Intenstinal Alkaline Phosphatase

Hintergrund: Background: At a high pH , Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research.

Gen-ID: 280993

UniProt: P19111