ADA Antikörper (Biotin)

Produktnummer ABIN1607721

Hersteller Produkt- Nr.: 200-406-140-0100

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ADA in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für ADA Antikörper (Biotin) (ABIN1607721)

Target: ADA (Adenosine Deaminase (ADA))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-ADA Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-406-140-0100

Hersteller: Rockland

Verwendungszweck: Adenosine Deaminase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].

Produktmerkmale: Synonyms: rabbit anti-Adenosine Deaminase Antibody biotin Conjugation, biotin conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Adenosine Deaminase [Calf Spleen]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Adenosine Deaminase Biotin Antibody has been tested by western blot. This product is suitable to be assayed against 1.0 μg of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:64,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu ADA

Target: ADA (Adenosine Deaminase (ADA))

Andere Bezeichnung: ADA

Hintergrund: Background: Adenosine Deaminase catalyzes the deamination of adenosine to inosine, thus destabilizing double-stranded RNA. Anti-Adenosine Deaminase is a member of the adenosine and AMP deaminases family. This antibody is a factor in purine metabolism and adenosine homeostasis, as well as positively regulating T-cell coactivation through the binding of DPP4. The antibody interacts with DPP4 in the extracellular domain, thus regulating lymphocyte-epithelial cell adhesion. It moderates extracellular adenosine signaling. Enzyme deficiency in Adenosine Deaminase has been associated with severe combined immunodeficiency disease, whereas an overabundance may result in congenital hemolytic anemia. Anti-Adenosine Deaminase is ideal for investigators involved in Enzyme, DNA and RNA research.

Gen-ID: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process