CPY Antikörper (Biotin)

Produktnummer ABIN1607713

Produktdetails anti-CPY Antikörper (Biotin)

Target: CPY (Carboxypeptidase Y (CPY))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPY Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.

Reinheit: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Carboxypeptidase Y [Baker’s Yeast]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Carboxypeptidase Y has been assayed against 1.0 µg of Carboxypeptidase Y in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu CPY

Target: CPY (Carboxypeptidase Y (CPY))

Andere Bezeichnung: Carboxypeptidase Y (CPY Produkte)

Synonyme: AO090103000332 antikoerper, carboxypeptidase Y antikoerper, CC1G_12159 antikoerper, PAS_chr1-4_0013 antikoerper, MCYG_00135 antikoerper, MGYG_00701 antikoerper, AFUA_5G14610 antikoerper, ANI_1_238044 antikoerper, AOR_1_542034 antikoerper

Hintergrund: Anti-Carboxypeptidase Y antibody is a participant in degrading small peptides. Carboyxpeptidase Y preferentially digests peptides containing aliphatic or hydrophobic residue in P1’ position, as well as methionine, leucine, or phenylalanine in P1 position of ester substrate. A member of the peptidase S10 family, Anti-Carboxypeptidase Y is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research. Synonyms: Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody

Gen-ID: 855343

UniProt: P00729