Dextranase Antikörper (Biotin)

Produktnummer ABIN1607709

Produktdetails anti-Dextranase Antikörper (Biotin)

Target: Dextranase

Reaktivität: Penicillium sp.

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Dextranase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Dextranase from other sources is unknown.

Reinheit: Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Dextranase [Penicillium species] DEX
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Dextranase has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Dextranase

Target: Dextranase

Abstract: Dextranase Produkte

Synonyme: dextranase antikoerper, Dextranase antikoerper, Teth39_0264 antikoerper, Teth514_0757 antikoerper, VDBG_04792 antikoerper, Thet_2161 antikoerper, Thebr_0271 antikoerper

Hintergrund: Anti-Dextranase antibody is a transferase enzyme which catalyzes the synthesis of dextran by engaging in the endohydrolysis of 1, 6 alpha-D-glucosidic linkages in dextran. Belonging to the glycosyl hydrolase 49 family, Anti-Destranase antibody is ideal for researchers interested in Metabolism and Signal Transduction research. Synonyms: Dextranase Alpha-1,6-glucan-6-glucanohydrolase DEX

UniProt: P48845