Glucose-6-Phosphate Dehydrogenase Antikörper (Biotin)

Produktnummer ABIN1607665

Hersteller Produkt- Nr.: 200-1653-0100

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper (Biotin)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Leuconostoc mesenteroides

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-1653-0100

Hersteller: Rockland

Verwendungszweck: Glucose-6-Phosphate Dehydrogenase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].

Produktmerkmale: Synonyms: goat anti-Glucose-6-Phosphate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody

Aufreinigung: Anti-Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Glucose-6-Phosphate Dehydrogenase Biotin has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Glucose-6-Phosphate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: Glucose-6-Phosphate Dehydrogenase (G6PD Produkte)

Hintergrund: Background: Anti-Glucose-6-Phosphate Dehydrogenase recognizes the oxidoreductase glucose-6-phosphate dehydrogenase. Found in the cytosol, glucose-6-phosphate dehydrogenase is responsible for oxidizing glucose-6-phosphate and reducing NADP to NADPH as part of the pentose phosphate pathway. As such, glucose-6-phosphate dehydrogenase is crucial in the maintenance of NADPH levels. A deficiency of glucose-6-phosphate dehydrogenase is a risk factor for non-immune hemolytic anemia. Glucose-6-phosphate dehydrogenase may also play a role in cell growth and proliferation and therefore, cancer.

Gen-ID: 6063509

UniProt: P11411

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones