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HCP Antikörper

Dieses Anti-HCP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HCP in WB und ELISA. Geeignet für E. coli.
Rockland
Produktnummer ABIN1607604
Hersteller Produkt- Nr.: 100-401-j07

Kurzübersicht für HCP Antikörper (ABIN1607604)

Target

HCP (Host Cell Proteins (HCP))

Reaktivität

  • 2
  • 1
  • 1
E. coli

Wirt

  • 4
Kaninchen

Klonalität

  • 4
Polyklonal

Konjugat

  • 4
Dieser HCP Antikörper ist unkonjugiert

Applikation

  • 4
  • 2
  • 1
Western Blotting (WB), ELISA
  • Hersteller Produkt- Nr.

    100-401-j07

    Hersteller

    Rockland

    Verwendungszweck

    Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.

    Spezifität

    E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins.

    Kreuzreaktivität (Details)

    Cross reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

    Produktmerkmale

    Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP

    Aufreinigung

    This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins.

    Sterilität

    Sterile filtered

    Immunogen

    Anti-Host Cell Proteins Antibody was prepared combining antibodies that have been separatly developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli.
    Immunogen Type: Native Protein
  • Applikationshinweise

    Western Blot Dilution: 1:500 - 1:1000
    ELISA : 1:1000-1:2000

    Kommentare

    Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    77.5 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store Host Cell Proteins Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    HCP (Host Cell Proteins (HCP))

    Andere Bezeichnung

    Host Cell Proteins
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