APP Antikörper

Produktnummer ABIN1589955

Dieses Anti-APP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von APP in IHC. Geeignet für Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für APP Antikörper (ABIN1589955)

Target: APP (Aminopeptidase P (APP))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (IHC)

Klon: JG12C9C10

Produktdetails anti-APP Antikörper

Verwendungszweck: Aminopeptidase P antibody

Spezifität: Membrane protein fraction of isolated rat glomeruli

Produktmerkmale: Chromosomal location: 1q55
Monoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated rat glomeruli as the immunizing antigen.

Aufreinigung: The mouse IgG1 antibody (#JG12C9C10) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Membrane protein fraction of isolated rat glomeruli

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handhabung: Centrifuge vial prior to opening.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Haltbarkeit: 24 months

Referenzen für anti-APP Antikörper (ABIN1589955)

Vlahu, Aten, de Graaff, van Veen, Everts, de Waart, Struijk, Krediet: "NEW INSIGHTS INTO THE EFFECTS OF CHRONIC KIDNEY FAILURE AND DIALYSATE EXPOSURE ON THE PERITONEUM." in: Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis, (2016) (PubMed).

Details zu APP

Target: APP (Aminopeptidase P (APP))

Andere Bezeichnung: Aminopeptidase P

Hintergrund: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble,Aminopeptidase P (AP-P, X-Pro aminopeptidase) has the unique ability to leave the N-terminal amino acid residue from peptides having proline as the penultimate amino acid residue. Biologically active peptides comprise an important and diverse class of extracellular chemical messengers that mediate a wide range of intercellular interactions. Several bioactive peptides including hormones, neuropeptides, neurotransmitters escape non-specific protease degradation by having an Xaa-Pro motif at their amino termini. Due to its cyclic nature, proline confers resistance to such peptide bonds so that aminopeptidases with broad specificity cannot act upon such peptides. There are a limited number of peptidases that act on peptide bonds involving a proline residue, such as dipeptidyl peptidase II (DPPII) and dipeptidyl peptidase W (DPPIV), and prolidase (which cleaves the Xaa-Pro bond only in dipeptides), or endopeptidases such as prolyl endopeptidase (which cleaves on the carbonyl side of proline residues within a protein or peptide). However,of these enzymes have been reported to hydrolyze Xaa-Pro bonds located at the N-terminus of peptides and proteins. Therefore, role of AP-P is crucial in this respect. AP-P activity is ubiquitous and has been found in a wide range of organisms including bacteria, yeast and vertebrates. Mammalian AP-Ps exist in membrane-bound and cytosolic forms, which represent two distinct gene products. The cytosolic (soluble) form of aminopeptidase P is found in human leukocytes and rat brain.

Gen-ID: 170751

NCBI Accession: NM_131913, NP_571988

UniProt: O54975