LYVE1 Antikörper (Biotin)

Produktnummer ABIN1589714

Der Kaninchen Polyklonal Anti-LYVE1-Antikörper wurde für FACS validiert. Er ist geeignet, LYVE1 in Proben von Maus zu detektieren.

Kurzübersicht für LYVE1 Antikörper (Biotin) (ABIN1589714)

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYVE1 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Produktdetails anti-LYVE1 Antikörper (Biotin)

Spezifität: Recombinant mouse soluble LYVE-1

Produktmerkmale: Chromosomal location: 7, 7F2
Produced from sera of rabbits immunised with highly pure recombinant mouse soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis). LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE 1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Aufreinigung: The antibody was purified via an antigen-affinity column and then biotinylated using a standard protocol.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FACS: use 3-10 µg/mL

Kommentare:

Rabbit IG Polyclonal Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Centrifuge vial prior to opening.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu LYVE1

Target: LYVE1 (Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE1))

Andere Bezeichnung: Lyve-1

Hintergrund: LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41 % similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Synonyms: Lyve1, Xlkd1, Lyve-1, Crsbp-1, 1200012G08Rik

NCBI Accession: NP_444477, NM_053247

UniProt: Q8BHC0

Pathways: Glycosaminoglycan Metabolic Process