Vimentin Antikörper

Produktnummer ABIN1580468

Der Maus Monoklonal Anti-Vimentin-Antikörper wurde für WB, IHC, IF und ICC validiert. Er ist geeignet, Vimentin in Proben von Human, Maus, Ratte, Schwein, Rind (Kuh) und Säugetier zu detektieren.

Kurzübersicht für Vimentin Antikörper (ABIN1580468)

Target: Vimentin (VIM)

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 2D1

Produktdetails anti-Vimentin Antikörper

Aufreinigung: affinity purified antibody

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Vimentin is a protein subunit of 10 nm or intermediate filaments, which are major components of the cytoskeleton in most cell types. Vimentin is the major intemediate filament subunit found in mesenchymal cells, and was first named and characterized in a collaborative study from the labs of German scientists Werner Franke and Klaus Weber. The name derives from the Latin Vimentum, meaning arrays of flexible rods such as in lattices, filigrees and wicker-work, which describes the intermediate filament network quite well. Vimentin is also found in many cell types in tissue culture, most notably fibroblasts, and in developing neuronal and astrocytic precursor cells in the central nervous system. Many cell lines such as Hek293, HeLa, 3T3 and Cos cells contain prominent vimentin networks. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in astrocytes, ependymal cells and neural stem cells), with desmin (in muscle and endothelial cells) and neurofilament proteins (in developing neurons). A E151K point mutation in the vimentin gene was shown to be causative of an autosomal dominant pulverulent cataract disease, but so far only in a single patient. Vimentin is a major protein of eye lens and cornea, and this mutation renders the molecule unable assemble into normal 10 nm filaments. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli. The same immunogen was used to produce our other monoclonal antibody to vimentin MCA-2A52. We also market a very popular chicken polyclonal antibody to vimentin, CPCA-Vim. The HGNC name for this protein is VIM.

Pathways: Caspase Kaskade in der Apoptose