Doublecortin Antikörper

Produktnummer ABIN1580415

Dieses Anti-Doublecortin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Doublecortin in WB, IHC, IF und ICC. Geeignet für Human, Maus, Ratte, Rind (Kuh), Schwein und Säugetier.

Kurzübersicht für Doublecortin Antikörper (ABIN1580415)

Target: Doublecortin (DCX)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Doublecortin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 3E1

Produktdetails anti-Doublecortin Antikörper

Aufreinigung: affinity purified antibody

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu Doublecortin

Target: Doublecortin (DCX)

Andere Bezeichnung: DCX Doublecortin

Hintergrund: Doublecortin was originally discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neuronal migration during development. The name Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. The HGNC name for Doublecortin is DCX, and it is also known as Doublin, Lissencephalin-X, DBCN and Lis-X. Four proteins encoded by the DCX produce bands of about 35 kDa and 45 kDa on Western blots (see the Uniprot page here). The 45 kDa form is known as Lis-XA while the smaller forms are generated by alternate transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these protein contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. The doublecortin protein appears to function as a microtubule and actin binding protein and may interact with Lis-1, a member of the beta,-transducin or WD protein family, a protein mutations of which are also associated with lissencephaly. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN, a neuronal specific protein characteristic of more mature neurons, now known to correspond to the RNA binding protein Fox3. Antibodies to DCX are used to identify stem cells in sections and in tissue culture, and to see if neurogenesis is taking place. Our antibody stains identically to the Doublecortin (C18): sc-8066 polyclonal peptide antibody available from Santa Cruz. The HGNC name for this protein is DCX.