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Avi-Tag Antikörper

WB, ELISA, IF, ICC, FACS Wirt: Maus Monoclonal 1D11D10 unconjugated
Produktnummer ABIN1574261
  • Target Alle Avi-Tag Produkte
    Avi-Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 5
    • 2
    Maus
    Klonalität
    • 5
    • 2
    Monoklonal
    Konjugat
    • 5
    • 1
    • 1
    Dieser Avi-Tag Antikörper ist unkonjugiert
    Applikation
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Sequenz
    GLNDIFEAQKIEWHE
    Spezifität
    Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.
    Kreuzreaktivität (Details)
    Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.
    Aufreinigung
    Protein A affinity column
    Immunogen
    Avi peptide conjugated - KLH
    Klon
    1D11D10
    Isotyp
    IgG2a
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Western blot: 0.2-1 µg/mL FACS: 1-3 µg for 1 x 106 cells ICC/IF: 1-3 µg/mL ELISA: 0.02-0.05 µg/mL
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Nogal, McCoy, van Gils, Cottrell, Voss, Andrabi, Pauthner, Liang, Messmer, Nedellec, Shin, Turner, Ozorowski, Sanders, Burton, Ward: "HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite." in: Science advances, Vol. 6, Issue 23, pp. eaba0512, (2020) (PubMed).

    Sheridan, Fong, DAlessandro, Bentley: "Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate." in: Molecular cell, Vol. 73, Issue 1, pp. 107-118.e4, (2019) (PubMed).

    Hill, Martinko, Nguyen, Wells: "Human antibody-based chemically induced dimerizers for cell therapeutic applications." in: Nature chemical biology, Vol. 14, Issue 2, pp. 112-117, (2019) (PubMed).

    Iovino, Orihuela, Moorlag, Molema, Bijlsma: "Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis." in: PLoS ONE, Vol. 8, Issue 7, pp. e68408, (2014) (PubMed).

  • Target
    Avi-Tag
    Andere Bezeichnung
    Avi Tag (Avi-Tag Produkte)
    Substanzklasse
    Tag
    Hintergrund
    Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.
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