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Protein C Tag Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Protein C Tag in WB und ELISA. Er zeigt eine Reaktivität gegenüber und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN1573910

Kurzübersicht für Protein C Tag Antikörper (ABIN1573910)

Target

Protein C Tag

Reaktivität

Bitte anfragen

Wirt

  • 2
  • 1
Kaninchen

Klonalität

  • 2
  • 1
Polyklonal

Konjugat

  • 3
Dieser Protein C Tag Antikörper ist unkonjugiert

Applikation

  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA
  • Spezifität

    This Antibody recognizes C-terminal, N-terminal, and internal Protein C tagged fusion proteins.

    Aufreinigung

    Immunoaffinity chromatography

    Immunogen

    Protein C epitope tag peptide CEDQVDPRLIDGK conjugated - KLH

    Isotyp

    IgG
  • Applikationshinweise

    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Buffer

    PBS, pH 7.4, containing 0.02 %Sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wong, Bienzle, Hayes, Taylor, Wood: "Purification of protein C from canine plasma." in: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).

    Wolf, Reimer, Schuck, Rüder, Gerlach, Müller, Otto, Dörken, Rehm: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).

  • Target

    Protein C Tag

    Substanzklasse

    Tag

    Hintergrund

    Rabbit Anti-Protein C (HPC4)-tag is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% Sodium azide.
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