VSV-g Tag Antikörper

Produktnummer ABIN1573888

Dieses Anti-VSV-g Tag-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von VSV-g Tag in WB, ELISA und IP. Geeignet für Vesicular Stomatitis Virus (VSV). Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für VSV-g Tag Antikörper (ABIN1573888)

Target: VSV-g Tag (VSV-G Epitope Tag)

Reaktivität: Vesicular Stomatitis Virus (VSV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VSV-g Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-VSV-g Tag Antikörper

Sequenz: YTDIEMNRLG K

Spezifität: This Antibody recognizes C-terminal, N-terminal, and internal VSV-G tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Immunogen: VSV-G epitope tag peptide YTDIEMNRLGK conjugated - KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc. The suitability of this antibody for applications other than thoselisted below has not been determined. The following concentration rangesare recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunoprecipitation (IP): 2-10 µg/mg of lysateOther applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-VSV-g Tag Antikörper (ABIN1573888)

Schmid, Sachs, tenOever: "Mitogen-activated protein kinase-mediated licensing of interferon regulatory factor 3/7 reinforces the cell response to virus." in: The Journal of biological chemistry, Vol. 289, Issue 1, pp. 299-311, (2014) (PubMed).

Zhang, He, Zhou, Pang, Liu, Zhao, Chen: "In vitro inhibition of vesicular stomatitis virus replication by purified porcine Mx1 protein fused to HIV-1 Tat protein transduction domain (PTD)." in: Antiviral research, Vol. 99, Issue 2, pp. 149-57, (2013) (PubMed).

Kita, Bertolesi, Hehr, Johnston, McFarlane: "Neuropilin-1 biases dendrite polarization in the retina." in: Development (Cambridge, England), Vol. 140, Issue 14, pp. 2933-41, (2013) (PubMed).

Zhao, Dai, Liang, Liang: "Surface display of rice stripe virus NSvc2 and analysis of its membrane fusion activity." in: Virologica Sinica, Vol. 27, Issue 2, pp. 100-8, (2012) (PubMed).

Padmashali, Andreadis: "Engineering fibrinogen-binding VSV-G envelope for spatially- and cell-controlled lentivirus delivery through fibrin hydrogels." in: Biomaterials, Vol. 32, Issue 12, pp. 3330-9, (2011) (PubMed).

Details zu VSV-g Tag

Target: VSV-g Tag (VSV-G Epitope Tag)

Andere Bezeichnung: VSV-G-Tag

Substanzklasse: Tag

Hintergrund: Rabbit Anti-VSV-G-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide. It is purified by immunoaffinity chromatography.