Trx Tag Antikörper

Produktnummer ABIN1573883

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in WB und ELISA. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für Trx Tag Antikörper (ABIN1573883)

Target: Trx Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 4C12H10

Produktdetails

Spezifität: This antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.

Kreuzreaktivität (Details): Reactivity to other species is not tested yet.

Aufreinigung: Protein G affinity column

Immunogen: Purified recombinant Trx-tag fusion protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.1-1.0 µg/mL
Western blot: 1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 mL WB with 2ml of PBST).Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Trx Tag

Andere Bezeichnung: Trx-Tag

Substanzklasse: Tag

Hintergrund: Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.