Vimentin Antikörper (APC)

Produktnummer ABIN1558082

Der Maus Monoklonal Anti-Vimentin-Antikörper wurde für ICFC validiert. Er ist geeignet, Vimentin in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Vimentin Antikörper (APC) (ABIN1558082)

Target: Vimentin (VIM)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist konjugiert mit APC

Applikation: Intracellular Flow Cytometry (ICFC)

Klon: VI-RE-1

Produktdetails anti-Vimentin Antikörper (APC)

Verwendungszweck: Anti-Vimentin APC

Spezifität: The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.

Keine Kreuzreaktivität: Maus, Schwein

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Bacterially expressed full-length human vimentin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-Vimentin Antikörper (APC) (ABIN1558082)

Chen, Chang, Wu, Li, Yang, Chen, Hsu, Chen, Wu, Lee, Huang, Goan, Chou, Huang, Wu: "Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line." in: Anticancer research, Vol. 30, Issue 3, pp. 727-36, (2010) (PubMed).

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Vimentin,Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose