EIF3F Antikörper (AA 81-130)

Produktnummer ABIN1534899

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch EIF3F in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für EIF3F Antikörper (AA 81-130) (ABIN1534899)

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-EIF3F Antikörper

Bindungsspezifität: AA 81-130

Verwendungszweck: eIF3ε Antibody

Spezifität: EIF3ε Polyclonal Antibody detects endogenous levels of eIF3ε protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Andere Bezeichnung: EIF3F

Hintergrund:

Synonyms: EIF3F, EIF3S5, Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47

Background: function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,similarity:Belongs to the eIF-3 subunit F family.,similarity:Contains 1 MPN (JAB/Mov34) domain.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I, module B is composed of EIF3F, EIF3H, and EIF3M, and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.,

Gene Name: EIF3F

Protein Name: Eukaryotic translation initiation factor 3 subunit F

Molekulargewicht: 37 kDa

Gen-ID: 8665

OMIM: 603914

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization