DBF4 Antikörper (AA 10-59)

Produktnummer ABIN1534167

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DBF4 in WB, ELISA, IF und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Affe.

Kurzübersicht für DBF4 Antikörper (AA 10-59) (ABIN1534167)

Target: DBF4 (DBF4 Homolog (DBF4))

Reaktivität: Human, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DBF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-DBF4 Antikörper

Bindungsspezifität: AA 10-59

Verwendungszweck: Dbf4 Antibody

Spezifität: Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Andere Bezeichnung: DBF4

Hintergrund:

Synonyms: DBF4, ASK, DBF4A, ZDBF1, Protein DBF4 homolog A, Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1

Background: function:Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.,induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 DBF4-type zinc finger.,similarity:Contains 2 BRCT domains.,subunit:Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L.,tissue specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines.,

Gene Name: DBF4

Protein Name: Protein DBF4 homolog A

Molekulargewicht: 76 kDa

Gen-ID: 10926

OMIM: 604281

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication